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gap43 ps41  (R&D Systems)


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    R&D Systems gap43 ps41
    Figure 6: <t>GAP43</t> expression is downregulated in CGRP-positive neurons upon loss of adipose mTORC2. (A) Immunoblot analysis of inguinal WAT (iWAT) tissue from control and iAdRiKO mice two weeks after tamoxifen treatment. (n ¼ 6; 6). (B) Immunoblot analysis of iWAT tissue from control and iAdRiKO mice four weeks after tamoxifen treatment. (n ¼ 6; 6). (C) Immunoblot analysis of surgically denervated iWAT depot (denervation) compared to iWAT depot from sham-operated mice (sham). Neurofilament heavy polypeptide (NFH). (n ¼ 5; 5). (D) Representative image of a large nerve bundle in iWAT of control mice immunostained with growth-associated protein 43 <t>(GAP43)-pS41</t> and calcitonin gene-related peptide (CGRP). (N ¼ 11; 9). (E) Representative image of a large nerve bundle in iWAT of control mice immunostained with GAP43-pS41 and tyrosine hydroxylase (TH). (N ¼ 19; 11).
    Gap43 Ps41, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gap43+ps41/Phospho-GAP-43+(S41)+Antibody/pm36028121-114-40-42
    Average 90 stars, based on 2 article reviews
    gap43 ps41 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Adipose mTORC2 is essential for sensory innervation in white adipose tissue and whole-body energy homeostasis."

    Article Title: Adipose mTORC2 is essential for sensory innervation in white adipose tissue and whole-body energy homeostasis.

    Journal: Molecular metabolism

    doi: 10.1016/j.molmet.2022.101580

    Figure 6: GAP43 expression is downregulated in CGRP-positive neurons upon loss of adipose mTORC2. (A) Immunoblot analysis of inguinal WAT (iWAT) tissue from control and iAdRiKO mice two weeks after tamoxifen treatment. (n ¼ 6; 6). (B) Immunoblot analysis of iWAT tissue from control and iAdRiKO mice four weeks after tamoxifen treatment. (n ¼ 6; 6). (C) Immunoblot analysis of surgically denervated iWAT depot (denervation) compared to iWAT depot from sham-operated mice (sham). Neurofilament heavy polypeptide (NFH). (n ¼ 5; 5). (D) Representative image of a large nerve bundle in iWAT of control mice immunostained with growth-associated protein 43 (GAP43)-pS41 and calcitonin gene-related peptide (CGRP). (N ¼ 11; 9). (E) Representative image of a large nerve bundle in iWAT of control mice immunostained with GAP43-pS41 and tyrosine hydroxylase (TH). (N ¼ 19; 11).
    Figure Legend Snippet: Figure 6: GAP43 expression is downregulated in CGRP-positive neurons upon loss of adipose mTORC2. (A) Immunoblot analysis of inguinal WAT (iWAT) tissue from control and iAdRiKO mice two weeks after tamoxifen treatment. (n ¼ 6; 6). (B) Immunoblot analysis of iWAT tissue from control and iAdRiKO mice four weeks after tamoxifen treatment. (n ¼ 6; 6). (C) Immunoblot analysis of surgically denervated iWAT depot (denervation) compared to iWAT depot from sham-operated mice (sham). Neurofilament heavy polypeptide (NFH). (n ¼ 5; 5). (D) Representative image of a large nerve bundle in iWAT of control mice immunostained with growth-associated protein 43 (GAP43)-pS41 and calcitonin gene-related peptide (CGRP). (N ¼ 11; 9). (E) Representative image of a large nerve bundle in iWAT of control mice immunostained with GAP43-pS41 and tyrosine hydroxylase (TH). (N ¼ 19; 11).

    Techniques Used: Expressing, Western Blot, Control

    Related Articles

    Blocking Assay:

    Article Title: Adipose mTORC2 is essential for sensory innervation in white adipose tissue and whole-body energy homeostasis.
    Article Snippet: For immunofluorescent staining, sections were rehydrated and antigen retrieved by boiling sections in target retrieval Solution (Dako) for 20 min in a KOS Microwave HistoSTATION (Milestone Medical). .. Sections were blocked using Protein block serum-free readyto-use (Dako) and then incubated in primary antibody diluted in antibody diluent with background reducing component (Dako) overnight at 4 C. Primary antibodies against tyrosine hydroxylase (1:500, Millipore, Cat#AB1542), CGRP (1:200, Enzo, BML-CA1137-0100), GAP43-pS41 (1:200, R&D system, PPS006), Neurofilament heavy polypeptide (1:1000, Abcam, ab8135), Synaptophysin (1:200, Abcam, ab32127) and Adiponectin (1:500, Invitrogen, PA1-054) were used. .. After washing, sections were incubated in secondary antibody (1:500) in antibody diluent with background reducing component (Dako) for 1 h at room temperature.

    Incubation:

    Article Title: Adipose mTORC2 is essential for sensory innervation in white adipose tissue and whole-body energy homeostasis.
    Article Snippet: For immunofluorescent staining, sections were rehydrated and antigen retrieved by boiling sections in target retrieval Solution (Dako) for 20 min in a KOS Microwave HistoSTATION (Milestone Medical). .. Sections were blocked using Protein block serum-free readyto-use (Dako) and then incubated in primary antibody diluted in antibody diluent with background reducing component (Dako) overnight at 4 C. Primary antibodies against tyrosine hydroxylase (1:500, Millipore, Cat#AB1542), CGRP (1:200, Enzo, BML-CA1137-0100), GAP43-pS41 (1:200, R&D system, PPS006), Neurofilament heavy polypeptide (1:1000, Abcam, ab8135), Synaptophysin (1:200, Abcam, ab32127) and Adiponectin (1:500, Invitrogen, PA1-054) were used. .. After washing, sections were incubated in secondary antibody (1:500) in antibody diluent with background reducing component (Dako) for 1 h at room temperature.



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    gap43 ps41  (R&D Systems)
    90
    R&D Systems gap43 ps41
    Figure 6: <t>GAP43</t> expression is downregulated in CGRP-positive neurons upon loss of adipose mTORC2. (A) Immunoblot analysis of inguinal WAT (iWAT) tissue from control and iAdRiKO mice two weeks after tamoxifen treatment. (n ¼ 6; 6). (B) Immunoblot analysis of iWAT tissue from control and iAdRiKO mice four weeks after tamoxifen treatment. (n ¼ 6; 6). (C) Immunoblot analysis of surgically denervated iWAT depot (denervation) compared to iWAT depot from sham-operated mice (sham). Neurofilament heavy polypeptide (NFH). (n ¼ 5; 5). (D) Representative image of a large nerve bundle in iWAT of control mice immunostained with growth-associated protein 43 <t>(GAP43)-pS41</t> and calcitonin gene-related peptide (CGRP). (N ¼ 11; 9). (E) Representative image of a large nerve bundle in iWAT of control mice immunostained with GAP43-pS41 and tyrosine hydroxylase (TH). (N ¼ 19; 11).
    Gap43 Ps41, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gap43+ps41/Phospho-GAP-43+(S41)+Antibody/pm36028121-114-40-42
    Average 90 stars, based on 1 article reviews
    gap43 ps41 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 6: GAP43 expression is downregulated in CGRP-positive neurons upon loss of adipose mTORC2. (A) Immunoblot analysis of inguinal WAT (iWAT) tissue from control and iAdRiKO mice two weeks after tamoxifen treatment. (n ¼ 6; 6). (B) Immunoblot analysis of iWAT tissue from control and iAdRiKO mice four weeks after tamoxifen treatment. (n ¼ 6; 6). (C) Immunoblot analysis of surgically denervated iWAT depot (denervation) compared to iWAT depot from sham-operated mice (sham). Neurofilament heavy polypeptide (NFH). (n ¼ 5; 5). (D) Representative image of a large nerve bundle in iWAT of control mice immunostained with growth-associated protein 43 (GAP43)-pS41 and calcitonin gene-related peptide (CGRP). (N ¼ 11; 9). (E) Representative image of a large nerve bundle in iWAT of control mice immunostained with GAP43-pS41 and tyrosine hydroxylase (TH). (N ¼ 19; 11).

    Journal: Molecular metabolism

    Article Title: Adipose mTORC2 is essential for sensory innervation in white adipose tissue and whole-body energy homeostasis.

    doi: 10.1016/j.molmet.2022.101580

    Figure Lengend Snippet: Figure 6: GAP43 expression is downregulated in CGRP-positive neurons upon loss of adipose mTORC2. (A) Immunoblot analysis of inguinal WAT (iWAT) tissue from control and iAdRiKO mice two weeks after tamoxifen treatment. (n ¼ 6; 6). (B) Immunoblot analysis of iWAT tissue from control and iAdRiKO mice four weeks after tamoxifen treatment. (n ¼ 6; 6). (C) Immunoblot analysis of surgically denervated iWAT depot (denervation) compared to iWAT depot from sham-operated mice (sham). Neurofilament heavy polypeptide (NFH). (n ¼ 5; 5). (D) Representative image of a large nerve bundle in iWAT of control mice immunostained with growth-associated protein 43 (GAP43)-pS41 and calcitonin gene-related peptide (CGRP). (N ¼ 11; 9). (E) Representative image of a large nerve bundle in iWAT of control mice immunostained with GAP43-pS41 and tyrosine hydroxylase (TH). (N ¼ 19; 11).

    Article Snippet: Sections were blocked using Protein block serum-free readyto-use (Dako) and then incubated in primary antibody diluted in antibody diluent with background reducing component (Dako) overnight at 4 C. Primary antibodies against tyrosine hydroxylase (1:500, Millipore, Cat#AB1542), CGRP (1:200, Enzo, BML-CA1137-0100), GAP43-pS41 (1:200, R&D system, PPS006), Neurofilament heavy polypeptide (1:1000, Abcam, ab8135), Synaptophysin (1:200, Abcam, ab32127) and Adiponectin (1:500, Invitrogen, PA1-054) were used.

    Techniques: Expressing, Western Blot, Control